gnrh elisa kit  (Cusabio)

Journal: International Journal of Molecular Sciences

Article Title: Effects of Microplastics on the Central Reproductive Neuroendocrine System in a Sheep Model

doi: 10.3390/ijms27052316

Figure Lengend Snippet: Relative mRNA expression of gonadotropin-releasing hormone (GnRH) ( A , C ) (mean ± SEM) and GnRH protein levels ( B , D ) (mean ± SEM) in the mediobasal hypothalamus (MBH; A , B ) and preoptic area (POA; C , D ) of sheep treated with vehicle (C), a lower dose of microplastics (LD), or a higher dose of microplastics (HD). Significance of differences: * p < 0.05, *** p < 0.001.

Article Snippet: The GnRH concentration in the homogenates was determined using the Goat gonadotropin-releasing hormone, GnRH ELISA Kit (CSB-E13276G, Cusabio, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Expressing

Journal: Endocrine Connections

Article Title: Role of KNDy neurons in puberty onset in male offspring following prenatal androgen exposure

doi: 10.1530/EC-25-0209

Figure Lengend Snippet: Effect of PNA on serum levels of GnRH, LH, FSH, and kisspeptin 1 in male offspring at the onset of puberty. No significant differences were observed between groups ( P > 0.05).

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits were used to quantify serum concentrations of GnRH (CSB-E08037r; Cusabio, China), LH (CSB-E12654r; Cusabio, China), FSH (CSB-E06869r; Cusabio, China), and kisspeptin 1 (CSB-E13434r; Cusabio, China).

Techniques:

Journal: NPJ Biofilms and Microbiomes

Article Title: Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice

doi: 10.1038/s41522-025-00742-6

Figure Lengend Snippet: A Representative graphs illustrating the immunofluorescence of GnRH in various DEHP treatment groups and control groups. Scale bars: 50 µm. B Representative graphs demonstrating immunohistochemical analysis. Scale bars: 100 µm. C Statistical plots illustrating the immunofluorescence of GnRH in various DEHP treatment groups and control groups ( n = 6, Student’s t test, *** p < 0.001). D Bar chart displaying the alterations in GnRH, FSH, estradiol, and progesterone protein levels in the serum of different treatment groups and control groups ( n = 6, Student’s t test, ** p < 0.01; *** p < 0.001).

Article Snippet: ELISA kits were used to measure GnRH, PGE2, FSH, estradiol and progesterone of serum according to the manufacturer’s instructions (Elabscience Biotechnology Co., Ltd).

Techniques: Immunofluorescence, Control, Immunohistochemical staining

Journal: NPJ Biofilms and Microbiomes

Article Title: Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice

doi: 10.1038/s41522-025-00742-6

Figure Lengend Snippet: A Bacterial richness (Sobs) and B bacterial diversity (Faith’s PD) among all treatments ( n = 6, mean ± SE). C Non-metric multidimensional scaling (NMDS) ordinations analysis of bacterial community composition across all treatments. Differences in bacterial community composition (NMDS) of different treatments were determined by analysis of permutational multivariate analysis of variance (PERMANOVA; *** p < 0.001). D Multiple regression tree (MRT) analysis of effects of DEHP treatment and treated time on bacterial community composition. The R 2 , error, cross-validation error (CV Error), and standard error (SE) of MRT analysis were listed under the tree. E Percent stacked bar chart based on the relative abundances of bacterial phyla, showing hierarchical clustering along with DEHP treated and control groups on top of the graph. Color blocks on the right show phyla names, the key on the right show significant changes and their direction (upward and downward arrows for increased and reduced abundances, respectively) for each phylum as compared DEHP treatment with control group, in short-term and long-term respectively (Student’s t test, * p < 0.05; ** p < 0.01). F Random Forest (RF) mean predictor importance (percentage of increase of mean square error (MSE), increase in MSE%) of bacterial diversity indices (NMDS1, sobs and Faith’S PD) and phyla abundances as drivers for mouse pregnancy rate and levels of HPO axis-related hormones comprised of Follicle-Stimulating Hormone (FSH), GnRH, Estradiol, PGE2 and Progesterone. The accuracy importance measure was computed for each tree and averaged over the forest (5000 trees). Percentage increases in the MSE of variables were used to estimate the importance of these predictors, and higher MSE% values imply more important predictors. Significance levels are as follows: * p < 0.05; ** p < 0.01.

Article Snippet: ELISA kits were used to measure GnRH, PGE2, FSH, estradiol and progesterone of serum according to the manufacturer’s instructions (Elabscience Biotechnology Co., Ltd).

Techniques: Biomarker Discovery, Control

Journal: NPJ Biofilms and Microbiomes

Article Title: Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice

doi: 10.1038/s41522-025-00742-6

Figure Lengend Snippet: Depicted are the DEHP exposure can (1) activate hypothalamic astrocytes and coupled with perturbation secretion of neuregulin and reproductive hormones (PGE2, GnRH and FSH); (2) destabilize gut microbial stability by fluctuating GUS-related microbial taxa ( Streptococcus spp. Etc.) that accompanied by perturbation secretion of reproductive hormones (Estrogen); (3) damage ovary physiological structure and accompanied by perturbation secretion of reproductive hormones (Estrogen and Progesterone), and thereby disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis (MGHPO) that cause a decrease of pregnancy rate in mice. Diagram was created using BioRender.

Article Snippet: ELISA kits were used to measure GnRH, PGE2, FSH, estradiol and progesterone of serum according to the manufacturer’s instructions (Elabscience Biotechnology Co., Ltd).

Techniques: